Coding

Part:BBa_K4619003

Designed by: KaMing Li   Group: iGEM23_UCAS-China   (2023-10-09)


TcpP-LBD

This part codes for TcpP ligand binding domain(TcpP-LBD). TcpP-LBD is a single-domain protein that binds the human primary bile acid and could form a dimer when bound. This process is achieved by rewiring TcpP–TcpH from V. cholerae to E.coli*and fusing it with the CadC DNA binding domain to construct a chimeric receptor. When activated, TcpP-CadC forms a dimer and activates the pCadBA promoter, turning on the quorum-sensing cascade. Since TcpP LBD is a single-domain protein, it could be easily swapped into other ligand binding domains like nanobodies, promoting compatibility for the platform.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
protein